Direct measurements of natural planktonic bacterial community viability by flow cytometry.
نویسندگان
چکیده
A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 61 7 شماره
صفحات -
تاریخ انتشار 1995